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Dr. Roman joined the Department of Radiology
and have been establishing a physiological and molecular imaging
laboratory. The laboratory’s approach is to combine modern
physiological and molecular biological techniques with imaging
modalities. There are two main areas of research in the laboratory
which are NIH supported; using magnetic resonance to detect gene
expression in the heart and the development of magnetic resonance
techniques for imaging pancreatic beta cell and islet function.
In order to successfully conduct this
research, the laboratory requires an array of equipment which is
unique to a Radiology department and provides us the ability to
conduct studies ranging from the single cell to the whole animal. The
laboratory has a cell culture facility consisting of a biosafety hood,
cell incubator, centrifuges, and a Zeiss fluorescence microscope. The
biochemistry lab is equipped with a high speed centrifuge,
quantitative real-time PCR machine, UV spectrophotometers, a
microplate reader, and a gel documentation and analysis system along
with associated electrophoresis equipment. The animal physiology
laboratory is equipped to perform microsurgery with the aid of a Zeiss
surgical microscope, associated rodent ventilator and anesthesia
machine. In vivo hemodyamic measurements are made in mice using a 1.4F
Millar pressure/volume transducer which is inserted into the mouse
ventricle and data digitized and analyzed in real time. This is not
trivial as the mouse heart is only 120 mg with a heart rate of 600-700
bpm!
The primary imaging modality used is magnetic
resonance, both spectroscopy and imaging. We currently utilize the
4.7T animal scanner located in the magnetic resonance imaging and
spectroscopy laboratory headed by Dr. Greg Karczmar for all in vivo
cardiac and pancreatic imaging. Cellular imaging studies of isolated
rodent and human pancreatic islets are conducted on a 11.7T scanner
located at the University of Illinois at Chicago (see image below). We
are looking forward to the acquisition of a 9.4T animal scanner which
will allow us conduct molecular imaging studies not possible on our
current equipment.
Updated
Curriculum Vitae (pdf) |
EDUCATION
B.A. Biology, Illinois Wesleyan University, Illinois, 1985
M.S. Physiology, University of Illinois at Urbana-Champaign, Illinois,
1988
Ph.D. Physiology, University of Illinois at Urbana-Champaign, Illinois,
1992
RESEARCH INTERESTS magnetic
resonance to detect gene expression in the heart, development of magnetic
resonance techniques for imaging pancreatic beta cell and islet function
Awards and
Honors
1993-1992 Keck Scholar, W.M. Deck Center for Advanced Training and
Research in computational Biology. University of Pittsburgh, Carnegie
Mellon University/Pittsburgh Supercomputing Center
1995 W.M. Keck Center for Advanced Training and Research in Computational
Biology Travel Fellowship. Funds for travel to the Annual Meeting of the
Society of Magnetic Resonance in Medicine in Nice, France.
Refereed Publications
1. Dawson, M.J., Fletcher, E.S., Ingkanisorn, S.S., Kmiecik,
J.A., and Roman, B.B., 31P and 1H Nuclear Magnetic Resonance Spectroscipic
studies of contracture-inducing agents in frog skeletal muscle. Journal of
Physiology, 1989; 415:133.
2. Roman, B.B., Foley, J.M., Meyer, R.A., and A.P. Koretsky, contractile
and Metabolic Effects of Increased Creatine Kinase Activeity in Mouse
Skeletal Muscle. American Journal of Physiology, 1996; 270: C1236-1245.
3. Roman, B.B., and Koretsky, A.P.. The brain isoform of Creatine Kinase
Rescues the Contractile Defect in Mice Lacking the Muscle Isoform Despite
Lack of Localization to Myofibrils. J Biol Chem. 1997; 272(28):17790-4.
4. LaBella, J.J., Daood, M.J., Koretsky, A.P., Roman, B.B., Sieck, G.C.,
Wieringa, B., and Watchko, J.F. Absence of Myofibrillar Creatine Kinase
and Diaphragm Isometric Function During Repetitive Activation. Journal of
Applied Physiology, 1998; 84(4), 116-1173.
5. Slawson, S.S., Roman, B.B., Williams, D.S., and Koretsky, A.P. Cardiac
MRI of the Normal and Hypertrophied Mouse Heart. Magnetic Resonance in
Medicine, 1998; 39; 980-987.
6. Roman, B.B., Geenen, D.L., Leitges, M., and Buttrick, PlM. PKC-B is not
necessary for Cardiac Hypertrophy. American Journal of Physiology – Heart
and Circ, 2001; 280: H2264-H2270.
7. Montgomery, D.E., Wolska, B.M., Pyle, W.G., Roman, B.B., Dowell, J.C.,
Buttrick, PlM., Koretsky, A.P., Del Nido, P., and Solaro, R.J.
Alpha-Adrenergic Response and Myofilament Activity in Mouse Hearts Lacking
PKC Phosphorylation Sites on Cardiac TnI. Am J Physiol Heart Circ Physiol,
2002; 282: H2397-405.
8. Roman, B.B., Meyer, R.A., and Wiseman, R. Phosphocreatine Kinetics at
the Onset of Contraction in Skeletal Muscle of MM Creatine Kinase Knockout
Mice. Am J Physiol Cell Physiol, 2002; 283(6); C1776-83.
9. Eroglu, S., Gimi, B., Roman, B.B., Friedman, G., and Magin, R. NMR
Spiral Microcoils: Design, Fabrication, and Imaging. Concepts in Magnetic
Resonance, 2003; 17B:1-10.
10. Gimi, B., Eroglu, S., Leoni, L., Desai, T.A., Magin, R.L., and Roman,
B.B. NMR Sprial Surface Microcoils: Applications. Concepts in Magnetic
Resonance, 2003; 18B: 1-18.
11. Itani S.I., Tapscott E.B., Leitges M., McKinney R., Roman B.B.,
Buttrick, P.M., and Dohm G.L. Effect of Protein Kinase C Beta Knckout on
Insulin Signal Transduction in Transgnic Mice. Amer J Physiol, 2003; In
revision.
12. Roman B.B., Goldspink, P.H., Spaite, E., Urboniene, D., McKinney, R.,
Geenen, D.L., Solaro R.J., and Buttrick P.M. Inhibition of PKC
Phosphorylation of cTnI Improves Cardiac Performance In Vivo. American
Journal of Physiology: Heart, 2004 286: H2089-H2095.
13. Barjor G., Leoni L., Oberholzer J., Braun M., Avila J., Wang Y., Desai
T., Philipson L.H., Magin R.L., and Roman B.B. Functional MR Microimaging
of Pancreatic B-Cell Activation. Cell Transplantation the Regenerative
Medicine Journal, 2006; 15: 195-203.
14. Fan, X., Markiewicz, E., Haque, M., Zamora, M., Karczmar, G.S., Roman,
B.B. Open Birdcage Coil and Physiological Chamber for Mouse Cardiac
Imaging. Journal of Cardiovascular Magnetic Resonance, 2006; 8(1);
298-299.
15. Fan X., Markiewicz E.J., Zamora M., Karczmar G., Roman B.B. Comparison
and Evaluation of Mouse Cardiac MRI Acquired With Open Birdcage, Single
Loop Surface And Volume Birdcage Coils, 2006; 51; N451-N459.
16. LaFlamme K., Leoni L., Popat K., Markiewicz E.J., Roman B.B., Desai
T.A. Biocompatibility of Porous Alumina Biocapsules, 2006 (In Press).
Grant Support
Current
2003-2008 Imaging Pancreatic B-Cell Function by Magnetic Resonance
NIH 1 R01 EB0011828-01: $1,250, 000
Role: PI
2003-2008 NMR Detection of Gene Expressions
NIH 1 R01 HL0609961-01: $1,328,334
Role: PI
2005-2006 Quantitative and Fast MRI Imaging of Pancreas Cancer
Cancer Research Center Pilot Grant (PI: Pan)
University of Chicago $30,000
Role: Co-Investigator
Completed
1994-1997 National Institutes of Health Postdoctoral Fellowship (NRSA)
1999-2001 Protein Kinase C Effects on Senescent Heart Function
NIH 1R03AG016443-01 $100,000
Role: Co-Investigator
2002-2004 Magnetic Resonance Imaging of Beta-Cell Activation
ADA Innovation Award $100,000
Role: PI |
